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Vitamin B2 Test

This blood test measures vitamin B2 (riboflavin) to evaluate deficiency. Riboflavin helps the body convert food into energy and supports the health of skin, eyes, and mucous membranes.

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Typical turnaround
1-3 business days
Starting price
From $120
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Quick answer

The vitamin B2 test measures riboflavin, a water-soluble vitamin involved in energy production. Riboflavin works with other B vitamins to help cells use carbohydrates, fats, and proteins. It also supports the health of skin, eyes, and the lining of the mouth and throat. The test uses a blood sample and is ordered when a deficiency is suspected or when nutrition needs review.

What this test measures

Riboflavin, or vitamin B2, is a building block for two important coenzymes, FAD and FMN, that drive many reactions in energy metabolism. It also helps regenerate other nutrients, including certain forms of vitamin B. Because the body cannot store much of it, a regular dietary supply is needed.

Testing may measure plasma or serum riboflavin or, more often, the activity of an enzyme that depends on it. One common approach is the erythrocyte glutathione reductase activity coefficient, which rises when riboflavin status is poor. The test requires only a blood sample.

Deficiency is uncommon in people eating a varied diet, since riboflavin is found in dairy, eggs, lean meats, and many fortified foods. It can appear with poor intake, malabsorption, or increased needs. Symptoms may include cracked lips, a sore tongue or throat, and skin irritation, though these signs overlap with other conditions.

  • Plasma or serum riboflavin
  • Erythrocyte glutathione reductase activity coefficient
  • Flavin adenine dinucleotide (FAD)
  • Flavin mononucleotide (FMN)
  • Related B vitamin markers when ordered together
  • Reference range comparison for riboflavin status

Why people order this test

  • Evaluate suspected riboflavin deficiency
  • Investigate cracked lips or a sore tongue
  • Review nutrient status with poor intake or malabsorption
  • Check B vitamin status in restrictive diets
  • Follow up on symptoms affecting skin and mucous membranes
  • Assess nutritional status before discussing supplementation
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How to prepare

Fasting is often not required, but the laboratory may request it depending on the method. A blood sample is drawn from a vein, usually at the arm. Follow the preparation instructions supplied with the order.

Understanding your results

Results are generally reported within one to three business days. The report indicates whether riboflavin status is adequate or low based on the laboratory method. A healthcare provider interprets the result with diet and symptoms.

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Frequently Asked Questions

What is vitamin B2 used for?

Riboflavin helps the body convert food into energy and supports the health of skin, eyes, and the tissues lining the mouth and throat.

How is riboflavin status measured?

Some laboratories measure riboflavin directly, while others measure the activity of an enzyme that depends on it. Both approaches use a blood sample.

What foods contain riboflavin?

Dairy products, eggs, lean meats, leafy greens, and fortified cereals and grains are common sources.

Do I need to fast before this test?

Fasting may be requested by some laboratories. Follow the instructions included with your order.

Can riboflavin deficiency occur with a normal diet?

It is uncommon with a varied diet, but it can happen with poor intake, absorption problems, or increased nutritional needs.

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Reviewed by the LabOrdering Editorial Team

Every page on this site is reviewed for clarity, sourcing, and accuracy. We update our library as testing options, collection networks, and accreditation standards change.

Medical disclaimer. The information on LabOrdering.com is educational only and is not medical advice, diagnosis, or treatment. Reference ranges and clinical guidance vary between laboratories. Always consult a qualified healthcare provider about your own results and care.