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Total Testosterone Blood Test

The total testosterone test measures the overall amount of testosterone in the blood, including bound and unbound forms. It is used to check for levels that are too high or too low and to evaluate symptoms linked to low testosterone.

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Typical turnaround
1-3 business days
Starting price
From $49
Results
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Quick answer

The total testosterone test measures the total amount of testosterone circulating in the blood. This includes testosterone attached to proteins and the small unbound fraction. It is a common first test when symptoms suggest a hormone imbalance, such as low energy, reduced libido, or mood changes, and results are interpreted by a qualified healthcare provider.

What this test measures

Testosterone is a sex hormone produced mainly by the testes in men and by the ovaries and adrenal glands in women. Total testosterone captures both the protein-bound portion and the free portion. Because symptoms of low or high testosterone can overlap with other conditions, the test is used as one piece of a broader evaluation rather than a stand-alone answer.

A blood sample is drawn, usually in the morning when testosterone is at its peak. The laboratory reports a total testosterone value and a reference range that varies by age and sex. A single low or high result is often repeated, since sleep, stress, illness, and timing can shift the number from day to day.

Clinicians order total testosterone testing to look into low libido, fatigue, mood changes, fertility concerns, or signs of hormone excess. It may also be used to monitor a known condition. The result does not diagnose a disorder on its own; a qualified healthcare provider evaluates it with symptoms and other findings.

  • Total testosterone
  • Free testosterone (when added)
  • Sex hormone-binding globulin (SHBG)
  • Luteinizing hormone (LH)
  • Follicle-stimulating hormone (FSH)
  • Estradiol
  • Prolactin
  • Albumin

Why people order this test

  • Check for low testosterone
  • Investigate possible high testosterone
  • Evaluate low libido, fatigue, or mood changes
  • Assess fertility concerns
  • Monitor a known hormone condition
  • Establish a baseline before further testing
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How to prepare

Collection is usually done in the morning when testosterone is at its highest. Fasting may be requested if the test is combined with other panels. Follow the timing instructions provided by the laboratory.

Understanding your results

Results are typically available within one to three business days. The report shows the total testosterone level and its reference range, which varies by age and sex. A qualified healthcare provider interprets the value with symptoms and any related tests.

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Frequently Asked Questions

What does the total testosterone test measure?

It measures all testosterone in the blood, both the portion bound to proteins and the free portion, giving an overall level.

Why is morning collection preferred?

Testosterone levels are highest in the morning, so collecting then gives a more consistent and comparable result.

Can a single result be relied on?

A single value can vary with sleep, stress, and illness, so a provider may repeat the test to confirm an abnormal result.

Does this test measure free testosterone too?

No. It measures total testosterone. Free testosterone requires a separate test or a calculation using other values.

Do I need to fast?

Fasting is not always required, but it may be requested when the test is combined with other panels. Follow the laboratory's instructions.

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Every page on this site is reviewed for clarity, sourcing, and accuracy. We update our library as testing options, collection networks, and accreditation standards change.

Medical disclaimer. The information on LabOrdering.com is educational only and is not medical advice, diagnosis, or treatment. Reference ranges and clinical guidance vary between laboratories. Always consult a qualified healthcare provider about your own results and care.