Test library
ACTH Level Test
An ACTH test measures adrenocorticotropic hormone, the pituitary signal that tells the adrenal glands to produce cortisol. It is used to investigate adrenal or pituitary disorders and is ordered together with a cortisol measurement.
Order ACTH Test
Order online and visit a local collection site, or choose an at-home kit where available. No referral and no insurance required.
Order ACTH Test- Typical turnaround
- 1-3 business days
- Starting price
- From $49
- Results
- Confidential, online
Quick answer
The ACTH test measures the level of adrenocorticotropic hormone in the blood. ACTH is produced by the pituitary gland and directs the adrenal glands to release cortisol, a hormone involved in stress response, blood pressure, and metabolism. The test is usually interpreted with a cortisol result and can help identify whether an adrenal or pituitary problem is driving symptoms.
What this test measures
The pituitary gland releases ACTH in pulses, and the hormone travels through the bloodstream to the adrenal glands. There it stimulates cortisol production. Because the two hormones work as a feedback loop, measuring ACTH without cortisol often leaves the picture incomplete, so laboratories and clinicians frequently review them together.
Testing may be requested when someone reports persistent fatigue, unexplained weight changes, low blood pressure, or other symptoms that raise concern about adrenal function. It can also be part of an evaluation for Cushing syndrome, adrenal insufficiency, or a pituitary disorder. Some clinicians use it to follow a known condition over time.
Interpretation depends on context. A high ACTH with a low cortisol points in one direction, while a low ACTH with a high cortisol points in another. Results can be affected by the time of day, stress, and medications, so clinicians consider the whole clinical picture before drawing conclusions.
- Adrenocorticotropic hormone (ACTH), also called corticotropin
- Serum cortisol, often ordered at the same draw
- Aldosterone
- Renin
- DHEA sulfate (DHEA-S)
- 17-hydroxyprogesterone
- Sodium and potassium
Why people order this test
- Investigate persistent fatigue, weakness, or low blood pressure
- Evaluate suspected adrenal insufficiency
- Support the workup of Cushing syndrome
- Check pituitary function after an abnormal cortisol result
- Monitor a previously diagnosed adrenal or pituitary condition
- Clarify the cause of unexplained electrolyte imbalances
How to prepare
ACTH levels change throughout the day, so the laboratory may ask for a morning collection. Fasting may be requested depending on which additional tests are bundled with the order. Follow the timing instructions from the laboratory, and tell the ordering provider about current medications.
Understanding your results
Results are usually available within one to three business days. The report shows the ACTH level, which is interpreted together with cortisol and any related tests. Values outside the reference range do not diagnose a condition by themselves; a qualified healthcare provider evaluates them in context.
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Frequently Asked Questions
What does ACTH do?
ACTH signals the adrenal glands to release cortisol. It is part of a feedback loop that helps regulate blood pressure, metabolism, and the body's response to stress.
Why is a cortisol test ordered with ACTH?
The two hormones are interpreted together. The relationship between ACTH and cortisol helps clinicians understand whether an abnormality starts in the pituitary, the adrenal glands, or elsewhere.
When should the sample be collected?
ACTH is often collected in the morning because levels vary during the day. The laboratory provides the timing instructions for the specific order.
Do I need to fast?
Fasting may be requested when ACTH is bundled with other tests. Otherwise, follow the preparation instructions supplied with the order.
Can medications affect the result?
Yes, some medications can influence ACTH and cortisol levels. Sharing a complete medication list with the ordering provider supports accurate interpretation.
Ready to get tested?
Order online and visit a collection site near you, or choose a mail-in kit. No referral and no insurance required.
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